Sty LT2 Restriction of UNI Cassettes
The following cassettes are restricted by wild-type Salmonella typhimurium hosts
UNI-Cmr
UNI-Kan
UNI-Spec
Test (Vol.o,p.85): camR, rifR probes into various chromosomal loci in wt or r-m+ bgs.
probes
CAMC: chloramphenicol cassette targeting cheY
ACPS(C): chloramphenicol cassette targeting acpS
ACPS(R): rifampicin cassette targeting acpS
backgrounds
wt: TT22970 sty(LT2) $pKD46
r-m+: TT22971 sty(LT2) metA22 metE551 trpD2 ilv-452 leu- pro-(leaky) hsdLT6 hsdSA29 hsdB strA120 $pKD46
results

Strain

CAMC

ACPS(C)

ACPS(R)

TT22970

0

0

35

TT22971

31

9

47

 

 

 

 

Although the numbers are not very large, they are consistent with destruction of probe by endogenous restriction enzyme StySBI acting on a site in camR but not present in rifR.