Effects of L-Arabinose on Growth

I have traditionally used 20 mM L-Arabinose to induce lambda red on pKD46. I have also noticed that r-m+ strains grew exceptionally slow during linear transformation. I decided to trace the cause of this defect by comparing their growth along with that of wild type plus or minus ampicillin (used to hold the plasmid) and plus varying concentrations of L-Arabinose. Results: 20 mM L-Arabinose inhibits growth of the r-m+ strain and inhibits yield of both strains. Ampicillin exacerbates the growth inhibition of the r-m+ strain, but not of the wild type. Experiment and data.

-- Eric Kofoid


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