Host Restriction of UNI Cassettes

Our UNI CamR, KanR & SpecR cassettes all contain sites for endogenous LT2 restriction endonucleases. I tested the efficiency of transformation of these cassettes into a wild-type background (TT22970) and an r-m+ background (TT22971). My results are consistent with the hypothesis that UNI CamR cassettes are degraded by wild type LT2 when introduced by electroporation. Test description and data.

-- Eric Kofoid

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