Archive for May 18th, 2009

cfu/pfu calculation worksheet

Monday, May 18th, 2009

While doing an experiment recently, I found that I was doing a lot of guesswork to figure out how much I should dilute resuspended colonies to get nicely spaced single colonies for viable counts. Since I was doing the simple calculations a lot, I decided to put together a worksheet to do it for me.

I also added a panel that does the calculation the other direction –from # of colonies on a plate back to the original # of cells in the original culture.

Trivial? Yes.

Useful? Hopefully.

Accurate? Probably.



-- Doug