Comments for The RADIO

Comment on Amplification Levels & Copy Number from Solexa by kofoid

kofoid — Wed, 29 Jul 2009 04:53:21 +0000

Yes, you actually do see clusters of read-pairs which seem to define incipient, low-level or ephemeral inversions, inversion-duplications and possibly even TIDs (Tandem Inversion Duplications). I am writing a post which discusses this very subject, soon to be foisted on you!

Comment on Amplification Levels & Copy Number from Solexa by Stanley Maloy

Stanley Maloy — Tue, 28 Jul 2009 19:14:46 +0000

Although there are obvious reasons why they might not show up, you might predict that you would see some inversion duplications (or duplication inversions) in the sequences. Do you actually see these events?

Comment on cfu/pfu calculation worksheet by kofoid

kofoid — Thu, 28 May 2009 22:55:48 +0000

Excellent result — just what spreadsheets are for! Maybe we could cobble this into a useful CGI on our website?

Comment on Yally Pally to Doug by kofoid

kofoid — Wed, 01 Apr 2009 03:06:56 +0000

You may have noticed a subtle change in the header and text of this post.

Aelric has astutely pointed out that the Little Rascals (aka “Our Gang”) actually said “Yally Pally!” and not “Wolly Polly!” when congratulating each other for a well-earned triumph. Usage is not unlike three cheers — “Let’s give Alfalfa a ‘Yally Pally’ for saving Darla!” ” YALLY PALLY! YALLY PALLY! YALLY PALLY!”

You get the idea.

Comment on On John’s award by kimi

kimi — Thu, 12 Mar 2009 17:46:40 +0000

The picture came out nice

Comment on On John’s award by kofoid

kofoid — Thu, 12 Mar 2009 00:29:15 +0000

Yay, John!

Comment on Amplification & Adaptive Mutagenesis by kofoid

kofoid — Fri, 06 Mar 2009 06:25:54 +0000

Gentle People –

I think we’ve strayed a bit from science. Let’s just chalk this up to exuberance at the new toy, and get back to the job at hand. By the way, I stand by “magic” and “religion”. I would have thrown “Lamarckianism” and “Lysenkoism” in there, too, but the list would have been too long!

Comment on Amplification & Adaptive Mutagenesis by rothlab

rothlab — Fri, 06 Mar 2009 06:18:06 +0000

“amongst” … “AMONGST”??? What is this? Do we have a royal mole in the lab?

Comment on Amplification & Adaptive Mutagenesis by admin

admin — Fri, 06 Mar 2009 06:15:07 +0000

Dear “rothlab” — Don’t hide behind the anonymity of a generic username. You know who you are and should have the courage to speak openly as yourself. It’s especially ironic that you’d do this while championing free speech!

As far as your critique, you’re probably have a point. It is our blog and it really is writing amongst ourselve as we would speak amongst ourselves. It’s just that I’m a proponent of decorum, and “magic” and “religion” seemed a little … tawdry.

Comment on Amplification & Adaptive Mutagenesis by rothlab

rothlab — Fri, 06 Mar 2009 06:07:39 +0000

Loosen up, Admin! It’s our blog, we can talk the way we want. After all, the PattySue Club trashes us bad all the time.